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BioResource International Inc
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Broad Institute Inc
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Image Search Results
Journal: Nutrients
Article Title: Heat-Killed Lactobacillus delbrueckii subsp. lactis 557 Extracts Protect Chondrocytes from Osteoarthritis Damage by Reducing Inflammation: An In Vitro Study
doi: 10.3390/nu16244417
Figure Lengend Snippet: Effects of LDL557 extracts (LDL557) on SW1353 cell viability and monosodium iodoacetate (MIA, M)-induced cell death. The SW1353 cells were incubated for 24 h in the absence or presence of LDL557 extracts in the range of 3–200 μg/mL ( A ), or the cells were treated with 10 μg/mL LDL557 extracts for 24 h, followed by incubation with or without 5 μM MIA for an additional 24 h. Then, cell viability was determined via crystal violet staining ( B ). Data are presented as a percentage of the control group, accompanied by the mean ± standard deviation (S.D.) from three experiments. Statistically significant differences are indicated by * ( p < 0.05 compared with the untreated control group) and # ( p < 0.05 compared with the MIA-treated group). C = control untreated cells; M = MIA-treated cells; LDL557 = cells treated only with LDL557 extracts; M+LDL557 = cells treated with LDL557 extracts followed by treatment with MIA.
Article Snippet: The
Techniques: Incubation, Staining, Control, Standard Deviation
Journal: Nutrients
Article Title: Heat-Killed Lactobacillus delbrueckii subsp. lactis 557 Extracts Protect Chondrocytes from Osteoarthritis Damage by Reducing Inflammation: An In Vitro Study
doi: 10.3390/nu16244417
Figure Lengend Snippet: Oxidative stress of SW1353 cells treated with LDL557 extracts or MIA. The details of the treatment with LDL557 extracts and MIA are described in , and flow cytometry was conducted to analyze the levels of ROS ( A ), H 2 O 2 ( B ), and NO ( C ) using DCFH-DA, MitoPY1, and DAF-FM DA staining, respectively. The untreated control is depicted by the color-filled area (yellow, blue, or green), while the treated groups are represented by the thick black lines. Data are presented as a percentage of the control group, accompanied by the mean ± standard deviation (S.D.) from three experiments. Statistically significant differences are indicated by * ( p < 0.05 compared with the untreated control group). C = control untreated cells; M = MIA-treated cells; LDL557 = cells treated only with LDL557 extracts; M+LDL557 = cells treated with LDL557 extracts followed by treatment with MIA.
Article Snippet: The
Techniques: Flow Cytometry, Staining, Control, Standard Deviation
Journal: Nutrients
Article Title: Heat-Killed Lactobacillus delbrueckii subsp. lactis 557 Extracts Protect Chondrocytes from Osteoarthritis Damage by Reducing Inflammation: An In Vitro Study
doi: 10.3390/nu16244417
Figure Lengend Snippet: Effects of LDL557 extracts or MIA on inflammatory and anti-inflammatory cytokines’ mRNA expression in SW1353 cells. The details of the treatment with LDL557 extracts and MIA are described in . The mRNA expression levels of IL-1β ( A ), IL-6 ( B ), TNF-α ( C ), and IL-10 ( D ) were analyzed via RT-qPCR with β-actin used as the internal control. Data are presented as a fold change of the control group, accompanied by the mean ± standard deviation (S.D.) from three experiments. Statistically significant differences are indicated by * ( p < 0.05 compared with the untreated control group) and # ( p < 0.05 compared with the MIA-treated group). C = control untreated cells; M = MIA-treated cells; LDL557 = cells treated only with LDL557 extracts; M+LDL557 = cells treated with LDL557 extracts followed by treatment with MIA.
Article Snippet: The
Techniques: Expressing, Quantitative RT-PCR, Control, Standard Deviation
Journal: Nutrients
Article Title: Heat-Killed Lactobacillus delbrueckii subsp. lactis 557 Extracts Protect Chondrocytes from Osteoarthritis Damage by Reducing Inflammation: An In Vitro Study
doi: 10.3390/nu16244417
Figure Lengend Snippet: Effects of LDL557 extracts or MIA on COX-2 and mPGES-1 mRNA expression in SW1353 cells. The details of the treatment with LDL557 extracts and MIA are described in . The mRNA expressions of COX-2 ( A ) and mPGES-1 ( B ) were measured with β-actin used as the internal control. Data are presented as a fold change of the control group, accompanied by the mean ± standard deviation (S.D.) from three experiments. Statistically significant differences are indicated by * ( p < 0.05 compared with the untreated control group). C = control untreated cells; M = MIA-treated cells; LDL557 = cells treated only with LDL557 extracts; M+LDL557 = cells treated with LDL557 extracts followed by treatment with MIA.
Article Snippet: The
Techniques: Expressing, Control, Standard Deviation